64-bit version 2010b Search Results


scientificsoftware prism  (Oxford Instruments)
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Oxford Instruments scientificsoftware prism
Scientificsoftware Prism, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab 2010b  (MathWorks Inc)
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64 bit installation of matlab version 7.11  (MathWorks Inc)
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64-bit version 2010b  (MathWorks Inc)
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64 Bit Version 2010b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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64-bit installation of  (MathWorks Inc)
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zen software 2010b sp1 (release version 6.0 for lsm710)  (Carl Zeiss)
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Carl Zeiss zen software 2010b sp1 (release version 6.0 for lsm710)
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Zen Software 2010b Sp1 (Release Version 6.0 For Lsm710), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nextseq 500  (Illumina Inc)
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Illumina Inc nextseq 500
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Nextseq 500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zen 2010b sp1 for zeiss 710  (Carl Zeiss)
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Carl Zeiss zen 2010b sp1 for zeiss 710
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Zen 2010b Sp1 For Zeiss 710, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2014 sp1  (Carl Zeiss)
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Carl Zeiss 2014 sp1
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2014 Sp1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zen software version 2014 sp1  (Carl Zeiss)
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Zen Software Version 2014 Sp1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prism version 6.0d  (GraphPad Software Inc)
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Prism Version 6.0d, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spinning disk (sd) microscopy  (Carl Zeiss)
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Carl Zeiss spinning disk (sd) microscopy
(A) Three confocal planes of a PGC plasma membrane labeled with Farnesylated-mCherry providing a z-axis view of invaginations extending into the cell interior. One such invagination is highlighted with a red arrowhead at each of the 3 planes. Note that in the first plane, the connection of the structure to the membrane is not clearly seen, such that it resembles a vesicle. Additional invaginations connected to the plasma membrane are marked with black asterisks. (B) The graph shows the number of membrane invaginations in wild-type PGCs and in PGCs with elevated contractility (CA-MLCK), with an example for a manipulated cell presented on the right (n= 22 and 29 cells from 6 and 19 embryos, respectively. The graph shows the combined data from two independent experiments). The red dashed line highlights the contour of a large travelling bleb with black asterisks marking membrane invaginations within the cell body (see also Movie S4D). The P value is calculated using non-parametric Mann Whitney test. (C) Localization of PGCs for the correlative electron <t>microscopy</t> analysis, employing an arrowhead-shaped laser mark (asterisk). The dashed area labels one of the PGCs studied in the Teneo VolumeScope (right panel). (D) The Teneo VolumeScope data was used to measure the extent of extra plasma membrane stored in invaginations and wrinkles in PGCs (excluding long membrane tubes that span multiple planes). Every 200nm, the circumference of the cell was outlined, either by following the invaginations and wrinkles precisely, or by crude tracing of the cell circumference (see example in the magnified region at the bottom). The ratio between the circumference lengths obtained using the detailed Teneo VolumeScope data and the coarse estimate for two cells is presented (29 and 15 sections were used for example A and B respectively). Scale Bars = 5 μm. See also Figure S2.
Spinning Disk (Sd) Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Key Resources Table

Journal: Developmental cell

Article Title: Bleb expansion in migrating cells depends on supply of membrane from cell surface invaginations

doi: 10.1016/j.devcel.2017.10.030

Figure Lengend Snippet: Key Resources Table

Article Snippet: ZEN software, versions 2010B SP1 (Release version 6.0 for LSM710) and 2014 SP1 (black edition, 64bit, Release version 9.0 for Lightsheet Z.1) , Zeiss , https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html.

Techniques: Recombinant, Electron Microscopy, Dominant Negative Mutation, Software

(A) Three confocal planes of a PGC plasma membrane labeled with Farnesylated-mCherry providing a z-axis view of invaginations extending into the cell interior. One such invagination is highlighted with a red arrowhead at each of the 3 planes. Note that in the first plane, the connection of the structure to the membrane is not clearly seen, such that it resembles a vesicle. Additional invaginations connected to the plasma membrane are marked with black asterisks. (B) The graph shows the number of membrane invaginations in wild-type PGCs and in PGCs with elevated contractility (CA-MLCK), with an example for a manipulated cell presented on the right (n= 22 and 29 cells from 6 and 19 embryos, respectively. The graph shows the combined data from two independent experiments). The red dashed line highlights the contour of a large travelling bleb with black asterisks marking membrane invaginations within the cell body (see also Movie S4D). The P value is calculated using non-parametric Mann Whitney test. (C) Localization of PGCs for the correlative electron microscopy analysis, employing an arrowhead-shaped laser mark (asterisk). The dashed area labels one of the PGCs studied in the Teneo VolumeScope (right panel). (D) The Teneo VolumeScope data was used to measure the extent of extra plasma membrane stored in invaginations and wrinkles in PGCs (excluding long membrane tubes that span multiple planes). Every 200nm, the circumference of the cell was outlined, either by following the invaginations and wrinkles precisely, or by crude tracing of the cell circumference (see example in the magnified region at the bottom). The ratio between the circumference lengths obtained using the detailed Teneo VolumeScope data and the coarse estimate for two cells is presented (29 and 15 sections were used for example A and B respectively). Scale Bars = 5 μm. See also Figure S2.

Journal: Developmental cell

Article Title: Bleb expansion in migrating cells depends on supply of membrane from cell surface invaginations

doi: 10.1016/j.devcel.2017.10.030

Figure Lengend Snippet: (A) Three confocal planes of a PGC plasma membrane labeled with Farnesylated-mCherry providing a z-axis view of invaginations extending into the cell interior. One such invagination is highlighted with a red arrowhead at each of the 3 planes. Note that in the first plane, the connection of the structure to the membrane is not clearly seen, such that it resembles a vesicle. Additional invaginations connected to the plasma membrane are marked with black asterisks. (B) The graph shows the number of membrane invaginations in wild-type PGCs and in PGCs with elevated contractility (CA-MLCK), with an example for a manipulated cell presented on the right (n= 22 and 29 cells from 6 and 19 embryos, respectively. The graph shows the combined data from two independent experiments). The red dashed line highlights the contour of a large travelling bleb with black asterisks marking membrane invaginations within the cell body (see also Movie S4D). The P value is calculated using non-parametric Mann Whitney test. (C) Localization of PGCs for the correlative electron microscopy analysis, employing an arrowhead-shaped laser mark (asterisk). The dashed area labels one of the PGCs studied in the Teneo VolumeScope (right panel). (D) The Teneo VolumeScope data was used to measure the extent of extra plasma membrane stored in invaginations and wrinkles in PGCs (excluding long membrane tubes that span multiple planes). Every 200nm, the circumference of the cell was outlined, either by following the invaginations and wrinkles precisely, or by crude tracing of the cell circumference (see example in the magnified region at the bottom). The ratio between the circumference lengths obtained using the detailed Teneo VolumeScope data and the coarse estimate for two cells is presented (29 and 15 sections were used for example A and B respectively). Scale Bars = 5 μm. See also Figure S2.

Article Snippet: 3 ′ nanos3UTR (Dominant Negative) This paper N/A RNA: 554: cd14-3 ′ nanos3UTR This paper N/A RNA: 947: zfrab5c (S36N)-3 ′ nanos3UTR (Dominant Negative) This paper N/A RNA: 949: zfrab11a (S25N)-3 ′ nos1 UTR (Dominant Negative) This paper N/A RNA: A700: egfp-zfvamp8- 3 ′ nos1 UTR This paper N/A RNA: A801: dsred-rab5c-3 ′ globin UTR This paper N/A RNA: A803: zfrab11a-3 ′ globin UTR This paper N/A RNA: A279: dsredex-[human beta 1, 4-galactosyltransferase]-3 ′ nos1UTR This paper N/A RNA: A741: dn-zfexo70-3 ′ nanos3UTR (Dominant Negative) This paper N/A RNA: A918: pa-gfp-3 ′ globin UTR This paper N/A RNA: B007: lifeact-Ruby- 3 ′ nanos3 UTR This paper N/A RNA: B790: ca-zfmlck-3 ′ nanos3 UTR This paper N/A RNA: B854: Δcxcr4b-egfp-3 ′ nanos3 UTR This paper N/A RNA: C229: cav1a-egfp-3 ′ nanos3 UTR This paper N/A RNA: C504: cav1a-egfp-3 ′ globin UTR This paper N/A RNA: C584: mcherry-clip170h-3 ′ nanos3 UTR This paper N/A RNA: C944: dendra2-f-3 ′ nanos3 UTR This paper N/A RNA: D333: dn-exo70-3 ′ globin UTR (Dominant Negative) This paper N/A RNA: D597: zfrab5c (S36N)-3 ′ globin UTR (Dominant Negative) This paper N/A RNA: D601: zfrab11a (S25N)-3 ′ globin UTR (Dominant Negative) This paper N/A RNA: D617: amph-nbar-yfp-3 ′ nanos3 UTR This paper N/A Software and Algorithms Graphpad GraphPad Software, La Jolla California USA https://www.graphpad.com/scientificsoftware/prism Imaris, version 8.4.1, 2016 Bitplane Inc. http://www.bitplane.com Fiji NIH https://fiji.sc ZEN software, versions 2010B SP1 (Release version 6.0 for LSM710) and 2014 SP1 (black edition, 64bit, Release version 9.0 for Lightsheet Z.1) Zeiss https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html VisiView, version 2.1.4, 2014 Visitron Systems GmbH http://www.visitron.de/Products/Software/VisiView/visiview.html Open in a separate window Key Resources Table Spinning Disk (SD) microscopy Embryos were imaged using Carl Zeiss Axio imager Z1 microscope equipped with Yokogawa csu10B spinning disk.

Techniques: Clinical Proteomics, Membrane, Labeling, MANN-WHITNEY, Electron Microscopy

Key Resources Table

Journal: Developmental cell

Article Title: Bleb expansion in migrating cells depends on supply of membrane from cell surface invaginations

doi: 10.1016/j.devcel.2017.10.030

Figure Lengend Snippet: Key Resources Table

Article Snippet: 3 ′ nanos3UTR (Dominant Negative) This paper N/A RNA: 554: cd14-3 ′ nanos3UTR This paper N/A RNA: 947: zfrab5c (S36N)-3 ′ nanos3UTR (Dominant Negative) This paper N/A RNA: 949: zfrab11a (S25N)-3 ′ nos1 UTR (Dominant Negative) This paper N/A RNA: A700: egfp-zfvamp8- 3 ′ nos1 UTR This paper N/A RNA: A801: dsred-rab5c-3 ′ globin UTR This paper N/A RNA: A803: zfrab11a-3 ′ globin UTR This paper N/A RNA: A279: dsredex-[human beta 1, 4-galactosyltransferase]-3 ′ nos1UTR This paper N/A RNA: A741: dn-zfexo70-3 ′ nanos3UTR (Dominant Negative) This paper N/A RNA: A918: pa-gfp-3 ′ globin UTR This paper N/A RNA: B007: lifeact-Ruby- 3 ′ nanos3 UTR This paper N/A RNA: B790: ca-zfmlck-3 ′ nanos3 UTR This paper N/A RNA: B854: Δcxcr4b-egfp-3 ′ nanos3 UTR This paper N/A RNA: C229: cav1a-egfp-3 ′ nanos3 UTR This paper N/A RNA: C504: cav1a-egfp-3 ′ globin UTR This paper N/A RNA: C584: mcherry-clip170h-3 ′ nanos3 UTR This paper N/A RNA: C944: dendra2-f-3 ′ nanos3 UTR This paper N/A RNA: D333: dn-exo70-3 ′ globin UTR (Dominant Negative) This paper N/A RNA: D597: zfrab5c (S36N)-3 ′ globin UTR (Dominant Negative) This paper N/A RNA: D601: zfrab11a (S25N)-3 ′ globin UTR (Dominant Negative) This paper N/A RNA: D617: amph-nbar-yfp-3 ′ nanos3 UTR This paper N/A Software and Algorithms Graphpad GraphPad Software, La Jolla California USA https://www.graphpad.com/scientificsoftware/prism Imaris, version 8.4.1, 2016 Bitplane Inc. http://www.bitplane.com Fiji NIH https://fiji.sc ZEN software, versions 2010B SP1 (Release version 6.0 for LSM710) and 2014 SP1 (black edition, 64bit, Release version 9.0 for Lightsheet Z.1) Zeiss https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html VisiView, version 2.1.4, 2014 Visitron Systems GmbH http://www.visitron.de/Products/Software/VisiView/visiview.html Open in a separate window Key Resources Table Spinning Disk (SD) microscopy Embryos were imaged using Carl Zeiss Axio imager Z1 microscope equipped with Yokogawa csu10B spinning disk.

Techniques: Recombinant, Electron Microscopy, Dominant Negative Mutation, Software